Competitive reverse transcription-polymerase chain reaction for quantitation of feline immunodeficiency virus
نویسندگان
چکیده
منابع مشابه
One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses.
A one-tube reverse transcription-polymerase chain reaction (RT-PCR) for absolute feline coronavirus (FCoV) quantitation was developed. The assay is based on the 5' nuclease activity of the Thermus flavus (Tfl) polymerase and a fluorogenic probe which generates fluorescence when it is cleaved. The fluorogenic probe, also called TaqMan(TM) probe (Perkin Elmer, Foster City, USA), is an oligonucleo...
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Background and Aims: Accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (FMD). In present study, was evaluated real time reverse transcription-polymerase chain reaction (rRT-PCR) assay along with diagnostic routine methods for the detection of all seven serotypes of FMD virus (FMDV), namely O, C, A, SAT1, 2, 3 and Asia 1 in biological sample...
متن کاملQuantitation of enteroviral RNA by competitive polymerase chain reaction.
The polymerase chain reaction (PCR) is a new diagnostic technique for the detection of enteroviral infection; however, it currently provides only qualitative results. The aim of this study was to adapt PCR for the accurate quantitation of enteroviral RNA in clinical specimens. For this purpose, we designed a standard RNA which was homologous to sequences at the 5' end of the coxsackie B3 entero...
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Background and objective:Millions of people in developing countries lose their lives due to acute respiratory infections, such as Influenza A & B and Adeno viruses. Given the importance of rapid identification of the virus, in this study the researchers attempted to design a method that enables detection of influenza A, B, and adenoviruses, quickly and simultaneously. ...
متن کاملAccuracy of polymerase chain reaction assays for diagnosis of feline immunodeficiency virus infection in cats.
OBJECTIVE To determine the sensitivity, specificity, and overall diagnostic accuracy of polymerase chain reaction (PCR) assays offered by commercial diagnostic laboratories for diagnosis of FIV infection in cats. DESIGN Prospective clinical trial. ANIMALS 124 cats. PROCEDURE Blood was collected from cats that were neither infected with nor vaccinated against FIV, uninfected cats that were...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 1995
ISSN: 0166-0934
DOI: 10.1016/0166-0934(94)00169-h